A field investigation was carried out at Eastern Block farm, Tamil Nadu Agricultural University, during winter season of 2011-12 to evaluate the new formulation of potassium salt based glyphosate (Roundup Crop Shield 460 SL) on seed cotton yield and quality parameters in winter irrigated cotton. Experimental results revealed that higher seed cotton yield was obtained with POE glyphosate at 1800 g/ha at 35 and 70 DAS with + IC at 45 and 55 DAS which was comparable with PE pendimethalin at 750 g/ha + IC at 45 and 55 DAS. While comparing, intercultural operation carried out treatments performed better and recorded more seed cotton yield than the treatments without intercultural operation, Cotton fibre quality in glyphosate applied plots was showed similarity with hand weeding, hand weeder weeding and power weeder weeding, shows that POE glyphosate application did not affect the quality parameters.