Fecundity study of earthworm species Eisenia foetida during vermicomposting of puncture wine weed (Tribulus terristris) mixed with cowdung in different combinations was carried out. It was observed that both weight gain and cocoon production was more when Tribulus terristris weed was mixed with pure cowdung. Net biomass gain by earthworm in different feed mixture was in order of C2>C4>C3>C5>C1 and that of cocoon production of C4>C2>C3>C5>C1. It indicated that Tribulus terristris weed is a good biomass and reproduction supporting medium which can be used effectively for culturing Eisenia foetida as well as recycling of weed material for production of vermicompost when mixed with pure cowdung. Therefore, there is a need to divert research activities to expore the potential of different weeds as a raw material for vermicomposting and their utilization and to design a national level policy for their proper utilization.